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. 2006 Mar 15;395(Pt 1):147–155. doi: 10.1042/BJ20051599

Figure 1. The phl gene is present as a single copy in different P. chrysogenum strains.

Figure 1

(A) P. chrysogenum DNA fragment cloned in phage F1.2 and in plasmid pZE1.2 (9.9 kb). The phl gene is indicated with a shaded arrow. The 0.9-kb phl fragment used as probe in hybridization experiments is indicated with a solid bar. Restriction endonuclease sites: A, ApaI; Ba, BamHI; C, ClaI; E, EcoRI; N, NotI; S, SalI; Sa, SacII; Sm, SmaI; St, StuI. (B) Hybridization of the DNA of three different strains of P. chrysogenum with the phl probe. Lane 1, parental Wis 54-1255 strain; lane 2, deletion mutant Wis npe10; lane 3, penicillin-overproducer AS-P-99 strain. The DNA was digested with different enzyme mixtures: S-B, SalI/BglII; Sm, SmaI; St-Sa, StuI/SacII. λM, size markers [λ bacteriophage digested with PstI hybridized with labelled λPst as probe (λP)]. (C) Hybridization of SmaI-digested DNA of the three P. chrysogenum strains with the phl (panel 1) or SalI-digested DNA of the three strains with the penDE (panel 2), showing that the phl gene is present in the three strains, whereas the penDE gene is absent in the deletion mutant Wis npe10 and amplified (thick band) in strain AS-P-99. Lanes 1, 2, and 3, DNA of the three P. chrysogenum strains as in (B).