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. 2006 Mar 15;395(Pt 1):147–155. doi: 10.1042/BJ20051599

Figure 3. Amplification of the phl gene results in increased benzylpenicillin production.

Figure 3

(A) Construction (pAMF9E) used to amplify the phl gene in an autonomous replicating plasmid containing the AMA1 sequence (ars) from Asp. nidulans. Restriction endonuclease sites: E, EcoRI; S, SalI; and Sm, SmaI. (B) HPLC of benzylpenicillin measured directly in culture broths of the parental strain (Wis, solid line) and the PhlAR+ transformant (broken line). (C) Direct HPLC determination of benzylpenicillin in culture broths of the parental Wis 54-1255 strain (solid line) and the disrupted Dphl1 mutant (broken line). Benzylpenicillin was eluted at 23 min. Note the significant decrease in penicillin production in the disrupted mutant. The second mutant DPhl2 showed the same reduction in penicillin production as DPhl1 (results not shown).