Table 4. Benzylpenicillin production at 72 and 96 h in culture broths in DF medium of the parental strain Wis 54-1255 and the DPhl1 transformant disrupted in the phl gene.
Data for penicillin concentrations are means±S.D. for three separate flasks.
| Benzylpenicillin production (μg/mg dry weight) | |||
|---|---|---|---|
| Strain | PAA concentration (g/l) | 72 h | 96 h |
| Wis 54-1255 | 0.5 | 3.2±0.32 | 4.9±0.30 |
| DPhl1 | 0.5 | 2.8±0.25 | 4.0±0.22 |
| Wis 54-1255 | 1 | 3.7±0.20 | 5.1±0.15 |
| DPhl1 | 1 | 2.3±0.25 | 3.6±0.35 |
| Wis 54-1255 | 1.5 | 3.8±0.28 | 5.4±0.4 |
| DPhl1 | 1.5 | 2.3±0.20 | 3.4±0.25 |