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. 2006 Feb 16;25(5):1046–1057. doi: 10.1038/sj.emboj.7600999

Figure 8.

Figure 8

14-3-3ɛ and DP-3 locate to the promoters of E2F target genes. (A) Chromatin immunoprecipilation (ChIP) was performed as described using chromatin prepared from SiHa cells pre- and postbleomycin treatment (5 μg/ml for 6 h) and primers encompassing the E2F sites in APAF-1, p73 and E2F-1 together with the indicated antibodies (see Materials and methods). β-Actin served as a negative control. (B) ChIP was performed as described using chromatin prepared from SiHa cells using the APAF-1 E2F binding site and 14-3-3ɛ antibody. Twice the amount of chromatin was used in (B) to that in (A). (C) Quantitation of 14-3-3ɛ binding by ChIP to the E2F sites in the APAF-1, p73 and E2F-1 promoters. Quantitation of the binding of 14-3-3ɛ to APAF-1 was taken from the results shown in (B).