Figure 5.

Increased sensitivity to proteasome inhibitors in LPS-stimulated I.29μ⁺ cells. (A) I.29μ⁺ cells, untreated or stimulated with LPS for 3 days, were cultured for 5 h in the presence of increasing concentrations of MG132, with or without the simultaneous addition of TM (2.5 μg/ml). The percentage of propidium iodide (PrId) positive cells determined by FACS was plotted after subtracting the value obtained without treatment. Mean±s.d. of three independent experiments. (B) TM alone did not significantly induce apoptosis, implying synergy with MG132 in inducing apoptosis in unstimulated I.29μ⁺ cells.