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. 2006 Feb 16;25(5):1137–1147. doi: 10.1038/sj.emboj.7600990

Figure 4.

Figure 4

Deletion of hda leads to decreased RNR levels in dnaNG157C but not in dnaAA345S and dnaAT174P mutant strains. (A) Equal amounts of total cellular protein from wild-type DHB4, dnaAA345S, dnaAT174P and dnaNG157C mutant strains harboring (+) or not (−) the hda gene were separated by SDS–15% PAGE. Subunit R2 (nrdB) of the RNR was detected by Western blotting. As loading control, cytoplasmic thioredoxin was also detected by Western blotting. (B) β-Galactosidase activity of the nrdA'–‘lacZ fusion in wild-type DHB4, dnaAA345S and dnaAT174P strains harboring (gray) or not (black) the hda gene.