Table 1.
Strains and plasmids used in this work
| Strains/plasmids | Relevant genotype | Reference |
|---|---|---|
| Plasmids | ||
| pDSW204 | ori pBR, ampR, IPTG inducible | Weiss et al (1999) |
| pDSW206 | ori pBR, ampR, IPTG inducible | Weiss et al (1999) |
| pBAD18kan | ori pBR, kanR, arabinose inducible | Guzman et al (1995) |
| pBAD43 | ori pSC101, spcR, arabinose inducible | Guzman et al (1995) |
| pSMG7 | pDSW204-nrdAB | Ortenberg et al (2004) |
| pSMG8 | pDSW204-nrdEF | This work |
| pSMG9 | pDSW204-nrdHIEF | This work |
| pSMG10 | pDSW204-nrdDG | This work |
| pSMG11 | pDSW204kan | This work |
| pSMG12 | pDSW204kan-nrdAB | This work |
| pSMG13 | pDSW204kan-nrdEF | This work |
| pSMG14 | pDSW204kan-nrdHIEF | This work |
| pSMG15 | pDSW204kan-nrdDG | This work |
| pSMG16 | pDSW206-dnaA | This work |
| pSMG17 | pDSW206-dnaAA345S | This work |
| pSMG18 | pDSW206-dnaAT174P | This work |
| pSMG19 | pBAD43-hda | This work |
| pZL411 | pET19b-His-dnaA | Li and Crooke (1999) |
| pHisDnaAA345S | pET19b-His-dnaAA345S | This work |
| pHisDnaAT174P | pET19b-His-dnaAT174P | This work |
| pTB101 | pBSoriC | Baker and Kornberg (1988) |
| M13mpRE85 | Fuller et al (1981) | |
| Strains | ||
| DHB4a | Δ(ara–leu)7697 araD139 ΔlacX74 galE galK rpsL phoR Δ(phoA)PvuII ΔmalF3 thi contains a mutation in dnaA leading to a P to S change in DnaA amino acid 18 relative to other wild-type strains (dnaAP18S) | Boyd et al (1987) |
| SMG218 | DHB4 dnaA…MiniTn10CmR | This work |
| SMG217 | DHB4 dnaAA345S…MiniTn10CmR | This work |
| SMG219 | DHB4 dnaNG157C…MiniTn10CmR | This work |
| SMG275 | DHB4 dnaAT174P…MiniTn10CmR | This work |
| MG1655 | Wild type | Jensen (1993) |
| SMG378 | MG1655 dnaAT174P… MiniTn10CmR | This work |
| SMG379 | MG1655 dnaAA345S…MiniTn10CmR | This work |
| SMG380 | MG1655 dnaNG157C…MiniTn10CmR | This work |
| SMG329 | DHB4 Δ(λattL-lom)∷bla nrdA'–'lacZ | This work |
| SMG334 | SMG329 dnaAA345S…MiniTn10CmR | This work |
| SMG337 | SMG329 dnaAT174P…MiniTn10CmR | This work |
| SMG340 | SMG329 dnaNG157C…MiniTn10CmR | This work |
| SMG300 | DHB4 pDSW204 | This work |
| SMG301 | DHB4 pDSW204-nrdAB | This work |
| SMG318 | SMG301 Δhda∷tetR | This work |
| BLR(DE3)pLysS | F− ompT hsdSB(rB−mB−) gal dcm Δ(srl-recA)306∷Tn10(TetR) λ(DE3) [pLysS(CamR)] | Novagen |
| aVariant of DHB4 that had been cured of the F′ plasmid was used throughout this study. | ||