. 2006 Mar 2;25(5):1148–1159. doi: 10.1038/sj.emboj.7601006

Table 4.

K_m values and relative V_max ratios (folded/linear Ea23 control) for gel-purified Euplotes G-quadruplexes stabilised in 50 mM monovalent cation and their linear counterparts

Primer	Stabilising cation	Conformation	K_m±s.d. (nM)	Relative V_max (folded/linear)
Ea23	K⁺	Linear/denatured	90±20^a	1.00±0.06
Oxy 3.5	K⁺	Linear/denatured	100±30	0.56±0.07
Oxy 3.5	K⁺	Intramolecular	460±120	0.30±0.03

Ea23	Na⁺	Linear/denatured	40±3	1.00±0.02
Oxy 3.5	Na⁺	Linear/denatured	47±12	0.65±0.03
Oxy 3.5	Na⁺	Intramolecular	27±9	0.56±0.03

Ea23	K⁺	Linear/denatured	110±40	1.00±0.14
Oxy 1.5	K⁺	Linear/denatured	40±7	0.67±0.03
Oxy 1.5	K⁺	Intermolecular	74±16	0.57±0.04
K_m's of Ea23 and G-quadruplexes were determined simultaneously to account for inter-day variation.