TABLE 1.
Injection of sheep with pBLV-IF encoding wild-type or D247G Taxa
| Expt no. | Injected BLV clone | Sheep no. | Sex | WBC/mm3 | PBL/mm3 | Age at injection (yr) | Provirus detectedb (wk) | Antibodies to BLVc
|
|
|---|---|---|---|---|---|---|---|---|---|
| gp51 | p24 | ||||||||
| 1 | Wild type | G250 | M | 8,000 | 6,960 | 1 | − | − | |
| G279 | M | 5,000 | 3,900 | 1 | 4 | + | − | ||
| G296 | M | 5,500 | 4,015 | 1 | 8 | − | − | ||
| D247G | G245 | M | 8,200 | 6,478 | 1 | − | − | ||
| G287 | M | 5,000 | 3,600 | 1 | 6 | + | − | ||
| G298 | M | 4,900 | 4,312 | 1 | − | − | |||
| pME18Neo (control) | G267 | M | 5,500 | 3,245 | 1 | − | − | ||
| G274 | M | 4,000 | 2,360 | 1 | − | − | |||
| 2 | Wild type | W109 | M | 10,500 | 7,980 | 1 | − | − | |
| W196 | M | 9,000 | 7,560 | 1 | − | − | |||
| B.0249 | M | 13,600 | 8,432 | 1 | − | − | |||
| D247G | W195 | F | 11,100 | 7,437 | 1 | 4 | + | − | |
| B.0234 | F | 15,700 | 13,816 | 1 | 6 | + | − | ||
| B.0240 | M | 9,000 | 6,930 | 1 | − | − | |||
| pBluescript II (control) | Or.111 | M | 9,300 | 6,789 | 1 | − | − | ||
a
Experimental injections were made in 1999 (experiment 1) and 2000 (experiment 2). WBC, white blood cells; PBL, peripheral blood lymphocytes.
b
Blood was periodically collected from each sheep, and total DNA was extracted from the blood and used as the template for PCR for detection of BLV proviral genomes, as described in Materials and Methods. The week at which the BLV proviral genome was first detected is indicated.
c
The BLV antibody in serum from sheep at 15 weeks after injection was detected by use of an immunodiffusion test as described in Materials and Methods. −, negative; +, positive.