Skip to main content
. 2006 Mar 23;116(4):1117–1126. doi: 10.1172/JCI26858

Figure 6. Suppression of JunB expression reverses anergy induction in Itch–/– T cells.

Figure 6

(A) Naive CD4+ T cells were isolated from Itch–/– mice using MACS beads, and the cells were cultured under Th2-differentiating conditions for 2 days. The cells were then infected with retrovirus derived from pSuper interfering RNA (RNAi) empty vector or pSuper JunB RNAi. Aliquots of cell lysates were immunoblotted with anti-JunB. The same membrane was reprobed with anti-actin. (B and C) The cells were then incubated with ionomycin for 16 hours, washed, and restimulated with anti-CD3 and anti-CD28. The cell proliferation (B) and IL-4 production (C) were measured.