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. Author manuscript; available in PMC: 2006 Oct 1.
Published in final edited form as: Exp Lung Res. 2005 Oct;31(8):783–806. doi: 10.1080/01902140500461026

Fig. 5.

Fig. 5

Comparison of mRNA expression levels of genes in normal and IPAH PASMC treated with or without BMP-2. Expression of mRNA for GAPDH, caveolin-2, c-Myc binding protein, c-Fos, annexin V, cytochrome c-1, and GATA-binding protein 2 was assessed by real-time PCR. A maximum of 45 cycles were used to detect mRNA levels, where a higher required cycle number indicates a lower mRNA level. Open bars show mRNA levels of various genes in control PASMC (not treated with BMP-2) while shaded bars represent data from PASMC treated with 200 nM BMP-2 for 24 hours. The mRNA expression level is reported as a function of cycle number for GAPDH controls (A), selected genes (caveolin-2, c-Myc binding protein, c-Fos) that are downregulated in normal PASMC but upregulated in IPAH PASMC (B), and selected genes (annexin V, cytochrome c-1, and GATA-binding protein 2) that are upregulated in normal PASMC but downregulated in IPAH PASMC (C) following BMP-2 exposure. ** P<0.01, * P<0.05 vs. untreated control cells (open bars).