Skip to main content
. 2002 Dec 30;100(1):364–369. doi: 10.1073/pnas.0136911100

Figure 1.

Figure 1

Absence of the gene Rpg1 in cv. Golden Promise. PCR amplification of seven Rpg1 gene fragments (lanes 1–7) with primer pairs covering the 4,919-bp gene region (primer sequences are given in Table 3, which is published as supporting information on the PNAS web site). Shown is DNA from Morex (A) and Golden Promise (B). Transformation plasmid pNRG040 has been constructed to express the Rpg1 gene in the susceptible cultivar Golden Promise. The genomic Rpg1 gene fragment with its complex exon (black box) and intron (gray line) structure under the control of the Rpg1 gene promoter (gray arrow) and terminator (gray box) was isolated from Morex and ligated into Agrobacterium vector pJH260.