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. 2002 Dec 30;100(1):364–369. doi: 10.1073/pnas.0136911100

Figure 4.

Figure 4

Southern blot analysis of T0 plants and individual T1 seedlings after determination of disease resistance. Immune T1 plants (HR) are observed in all three transgenic lines. The single Rpg1 gene copy of transformant H228.2c is sufficient to confer immunity against P. graminis f. sp. tritici pathotype MCC, which is also observed in the progeny plants of line H228.5 containing either one or two Rpg1 gene copies. T1 plants of line H228.19 with two to five copies displayed a wide range of ITs, probably because of the complex integration and segregation pattern.