Table 3.
RT-PCR verification of the differentially expressed genes on chromosome 11q22-q24 identified by microarray
| Gene | Gene symbol | GO Biological Process | Quantitive RT-PCR |
Microarray |
||
|---|---|---|---|---|---|---|
| Fold change (obese/non-obese) | p value | Fold change (obese/non-obese) | p value | |||
| Upregulated | ||||||
| C1q and tumor necrosis factor related protein 5 | C1QTNF5 | Inflammation/immune response | 3.8 | <0.00001 | 2.1 | <0.00001 |
| Thy-1 cell surface antigen | THY1 | Inflammation/immune response | 5.5 | <0.00001 | 1.8 | <0.00001 |
| Fasciculation and elongation protein zeta 1 (zygin I) | FEZ1 | Cell adhesion | 2.9 | <0.00001 | 3.0 | <0.00001 |
| Nicotinamide | NNMT | Nucleic acid metabolism | 2.6 | <0.00001 | 2.1 | <0.00001 |
| N-methyltransferase | ||||||
| Hypoxia up-regulated 1 | HYOU1 | Response to stress | 1.5 | 0.005 | 1.2 | <0.00001 |
| Transgelin | TAGLN | N/A | 3.0 | <0.00001 | 2.7 | <0.00001 |
| Downregulated | ||||||
| Immunoglobulin superfamily, member 4 | IGSF4 | Cell cycle control/cell proliferation | −2.5 | <0.00001 | −2.0 | <0.00001 |
| Vacuolar protein sorting 11 | VPS11 | Transport/carrier | 1.2 | 0.07 | −1.3 | <0.00001 |
| Acetyl-Coenzyme A acetyltransferase 1 | ACAT1 | Energy pathway, electron transport | 1.1 | 0.4 | −1.4 | <0.00001 |
| SNF1-like kinase 2 | SNF1LK2 | Protein modification | −1.1 | 0.4 | −1.7 | <0.00001 |
| Sterol-C5-desaturase-like protein | SC5DL | Lipid metabolism | −1.4 | 0.0009 | −1.6 | <0.00001 |
| Roundabout axon guidance receptor, homolog 3 | ROBO3 | N/A | −1.1 | 0.6 | −1.7 | <0.00001 |
N/A not applicable (no assigned GO Biological Process)