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. 2005 Dec;242(6):851–858. doi: 10.1097/01.sla.0000189571.84213.b0

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FIGURE 1. Generation and characterization of 3D cultures of HBL cells. HBL cells were cultured for 72 hours on polyHema-coated plates, thus giving rise to the formation of multicellular aggregates (A) of an average diameter of 400 to 500 μm. Alamar blue cell proliferation assays (B) show distinct kinetics for cells cultured in 2D or in 3D.