FIGURE 7. Modulation of gene expression in HBL cells cultured in 3D as compared with 2D. HBL cells were cultured in 2D or in 3D for 3 days. Total cellular RNA was then extracted, reverse transcribed, and labeled as described in Materials and Methods prior to hybridization to oligonucleotide chips. Only characterized genes displaying a ≥3-fold up- or down-regulation (fold change) in either culture conditions are reported. Statistical significance (P < 0.05) refers to duplicate readings of data obtained from 2 distinct hybridization experiments. A, Overall modulation of gene expression. B and C refer to genes up- regulated or down-regulated in 3D as compared with 2D cultures, respectively. Genes whose expression is modulated upon 3D as compared with 2D culture are listed in D.