FIG. 2.

Effect of a microinjected antibody against the TfR cytoplasmic tail on CPV infection of Cf2Th cells. Cells were injected with anti-TfR IgG or with a control IgG, inoculated with 2 TCID₅₀ of CPV type 2 per cell, and then incubated for 48 h. Injected cells were identified by staining for the IgG, and infected cells were identified by staining for the viral NS1 protein. The proportion of antibody-injected cells that became infected was compared to the proportion of noninjected cells infected in the same culture. Bars show one standard deviation of the mean for four experiments with the anti-TfR and for two experiments with the control IgG.