FIG. 2.

In vivo packaging assays of the NC mutants. (A) RIPA analysis of pelleted virus-like particles collected from supernatants to quantitate the number of viral particles released to the medium. QT6 cells represent untransfected cells. (B) RPA to measure the amount of neo RNA in cells transfected with either ΔCH2, K58A, or ΔPR mutation or supernatant virions, using an antisense neo RNA as the riboprobe. (C) RPA to measure the effect of the ΔCH1 mutation on the ability of Gag polyprotein to package viral RNA into virions. The amount of neo RNA was measured in transfected cells and virions.