TABLE 1.

Relative packaging efficiencies of NC mutants to ΔPR

Gag constructa	Viral Gagb	Ratio of viral to cellular RNAc	Relative packaging efficiencyd
ΔCH1	0.033 ± 0.01	0.026 ± 0.01	0.78 ± 0.08
ΔCH2	0.033 ± 0.01	0.164 ± 0.04	3.89 ± 0.95
K58A	0.21 ± 0.02	1.485 ± 0.11	6.59 ± 0.47
ΔPR	1	1	1

^aNC mutations were made in Gag polyproteins lacking the PR domain.

^bRIPA were performed three times, and a representative gel is shown in Fig. 2A. Gels were scanned by a Molecular Dynamics PhosphorImager, and radioactive bands were quantitated, in machine units, by using ImageQuant software. The averages ± standard deviations are shown.

^cRPAs were performed three times (Fig. 2B and C), and quantitation was done by PhosphorImager analysis. The averages ± standard deviations are indicated.

^dThe relative packaging efficiencies to ΔPR were summarized from Fig. 2. The averages ± standard deviations were calculated from three independent experiments.