Table 1.
Mutant | ΔΔGD–N | ΔΔGD–‡ | φexp |
---|---|---|---|
T15A | 1.39 ± 0.18 | 0.25 ± 0.01 | 0.2 ± 0.1 |
E32A | 1.08 ± 0.15 | 0.48 ± 0.08 | 0.4 ± 0.1 |
T34A | 0.85 ± 0.24 | 0.65 ± 0.12 | 0.8 ± 0.3 |
I37A | 0.17 ± 0.20 | — | — |
V40A | 0.11 ± 0.16 | — | — |
R44A | 0.35 ± 0.16 | — | — |
T45A | 1.04 ± 0.16 | 0.75 ± 0.25 | 0.8 ± 0.3 |
Free energy differences in kcal⋅mol−1.