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. 2003 Jan 8;100(2):438–442. doi: 10.1073/pnas.0237108100

Figure 2.

Figure 2

Restriction maps of HSV8-lsc genes for expression in C. reinhardtii chloroplasts. (A) Relevant restriction sites delineating the rbcL 5′ UTR (BamHI/NdeI), the HSV8 coding region and flag tag (NdeI/XbaI), and the rbcL 3′ UTR (XbaI/BamHI), as well as relevant restriction sites of the atpA 5′ UTR (BamHI/NdeI), the HSV8 coding region and flag tag (NdeI/XbaI), and the rbcL 3′ UTR (XbaI/BamHI) are shown. (B) Restriction map showing the site of integration of the HSV8-lsc genes into plasmid p322 for integration into the C. reinhardtii chloroplast genome. p322 DNA includes the 5.7-kb region from EcoRI to XhoI in the C. reinhardtii chloroplast genome corresponding to position 44,877–50,577 (www.biology.duke.edu/chlamy_genome/chloro.html). Double-headed arrows indicate regions corresponding to the probes used in the Southern blot analysis. Black boxes indicate, from left to right, psbA exon 5, and the 5S and a small portion of the 23S ribosomal RNA genes, respectively.