Time course of chromatographic peak areas of phosphopeptides normalized to LIEDNEpYTAR peak areas. CD3ζ Tyr-141 (♦), CD3δ Tyr-160 (■), CD3ɛ Tyr-199 (●), CD3ζ Tyr-122(+), CD3δ Tyr-149 (−), ZAP-70 Tyr-315,319 (▴), and ZAP-70 Tyr-493 (*). Profiles shown here are representative of all observed.