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. 2003 Jan 9;100(2):443–448. doi: 10.1073/pnas.2436191100

Figure 3.

Figure 3

ZAP-70 Tyr-315,319 phosphorylation in CD3/CD4-stimulated Jurkat cells compared with LIEDNEpYTAR phosphorylation. Total ion chromatogram (TIC) recorded during MS analysis of phosphorylated peptides from unstimulated Jurkat cells (a) and Jurkat cells stimulated with crosslinked anti-CD3 and anti-CD4 antibodies for 1 min (b). Selected-ion chromatogram (SIC) or plot of the ion current vs. time for m/z 990 corresponding to the (M + 3H)3+ of the phosphopeptide ZAP-70 Tyr-315,319 for unstimulated (c) and 1 min stimulated (d) Jurkat cells. SIC for m/z 742.6 corresponding to the (M + 4H)4+ of the phosphopeptide ZAP-70 Tyr-315,319 for unstimulated (e) and 1 min stimulated (f) Jurkat cells. SIC for m/z 686 corresponding to the (M + 2H)2+ of the phosphopeptide LIEDNEpYTAR for unstimulated (g) and 1 min stimulated (h) Jurkat cells.