Table 1.
Phosphorylated peptides from anti-CD3/CD4-stimulated Jurkat cells
| Protein | NCBI GI no. | Peptide sequence | PO4 site | Known PO4 site (ref.) | Top scansite hits (24) | 0 min | 0.5 min | 1 min | 2 min | 5 min |
|---|---|---|---|---|---|---|---|---|---|---|
| Jurkat | ||||||||||
| CAS-L | 5453680 | TGHGYVpYEYPSR | Y166 | J-0.32, Y-0.25, JJ-0.27 | x | |||||
| CBL | 115855 | IKPSSpSANAIpYSLAAR | S669, Y674 | Y674 (39) | S669:None,Y674:B-2.2 | x | ||||
| CD3δ | 4502669 | NDQVpYQPLR | Y149 | S,X-1.7,GG-2.8 | x | x | ||||
| CD3δ | 4502669 | DRDDAQpYSHLGGNWAR | Y160 | S,X-3.4,GG-4.9 | x | x | x | x | x | |
| CD3ɛ | 4502671 | DLpYSGLNQR | Y199 | Y199 (40) | S,X-0.36,GG-0.52 | x | x | x | x | x |
| CD3ζ | 115997 | NPQEGLpYNELQK | Y110 | Y110 (29) | T,N-0.22,X-0.02,II-0.83 | x | ||||
| CD3ζ | 115997 | RKNPQEGLpYNELQK | Y110 | Y110 (29) | T,N-0.22,X-0.02,II-0.83 | x | x | |||
| CD3ζ | 115997 | MAEApYSEIGMK | Y122 | Y122 (29) | T,P-1.6,Y-1.9,JJ-1.9 | x | x | |||
| CD3ζ | 115997 | GHDGLpYQGLSTATK | Y141 | Y141 (29) | U,X-0.24,Y-3.6,GG-0.76 | x | x | x | x | x |
| CD3ζ | 115997 | REEpYDVLDKR | Y83 | Y83 (29) | S,X-0.36,GG-1.5,II-1.9 | x | ||||
| CD5 | 7656965 | pSHAENPTASHVDNE pYSQPPRNpSR | S439, Y453, S460 | Y453 (41) | Y453:J-0.96,W-0.66,Z-0.47 S460:None,S439:None | x | ||||
| GADS | 4758476 | RHpTDPVQLQAAGR | T262 | A-1.0,D-4.7,EE-0.65 | x | x | x | |||
| HS1 | 4885405 | GFGGQpYGIQK | Y198 | None | x | x | ||||
| LIM lipoma | 5031887 | YYEGYpYAAGPGYGGR | Y301 | B-3.3,W-2.1 | x | |||||
| PYK2 | 4758976 | YIEDEDpYpYKASVTR | Y579,Y580 | Y579,Y580 (42) | Y579:L-1.6,O-1.4,Q-2.3 Y580:None | x | ||||
| ZAP-70 | 1177033 | RIDTLNSDGpYTPEPAR | Y292 | Y292 (20) | None | x | x | x | ||
| ZAP-70 | 1177044 | PMPMDTSVpYESPpYSDPE ELKDK | Y315,Y319 | Y315, Y319 (21) | Y315:C-0.30,W-0.12,Z-0.08 Y319:C-1.9,W-2.1,Z-1.6 | x | x | |||
| ZAP-70 | 1177044 | ALGADDSpYpYTAR | Y492,Y493 | Y492, Y493 (20) | Y492:None,Y493:None | x | ||||
| ZAP-70 | 1177044 | ALGADDSYpYTAR | Y493 | Y493 (20) | None | x | x | x |
Times are after anti-mouse IgG treatment (T cell receptor crosslinking). NA, time points not analyzed in the experiments. Scansite motifs are represented by a letter indicating the motif followed by a percentile score for that motif. A, 14-3-3 mode 1 site; B, ABL kinase; C, ABL SH2; D, AKT kinase; E, ATM kinase; F, casein kinase 2; G, Cdc2 kinase; H, Cdk5 kinase; I, Clk2 kinase; J, Crk SH2; K, DNA PK; L, EGFR kinase; M, Erk1 kinase; N, FGFR kinase; O, FGFR SH2; P, Fyn SH2; Q, Grb2 SH2; R, GSK3 kinase; S, ITAM 1; T, ITAM 2; U, ITAM 3; V, ltk kinase; W, ltk SH2; X, Lck kinase; Y, Lck SH2; Z, Nck SH2; AA, p85 SH2; BB, PDGFR kinase; CC, PDZ class II; DD, PLCg N-terminal SH2; EE, protein kinase A; FF, Shc PTB; GG, Shc SH2; HH, SHIP SH2; II, Src kinase; JJ, Src SH2.