Table 2.
Phosphorylated peptides from BCR/ABL-expressing cells treated with STI571
| Protein | NCBI GI no. | Peptide sequence | PO4 site | Known PO4 site (ref.) | Top scansite hits (24) | 0 hr | 1.5 hr | 3 hr |
|---|---|---|---|---|---|---|---|---|
| 32d p210 | ||||||||
| ABL | 125135 | LMTGDTpYTAHAGAK | Y393 | Y393 (43) | None | x | NA | |
| ABL | 125135 | LMTGDTpYpTAHAGAK | Y393, T394 | Y393 (43) | Y393:None,Y394:None | x | NA | |
| ABL | 125135 | LpYVSSESR | Y185 | None | x | x | NA | |
| ABL | 125135 | pYSLTVAVK | Y264 | Y264 (44) | None | x | NA | |
| ABL | 125135 | NKPTVpYGVSPNYDK | Y226 | Y226 (43) | B-3.3,X-3.4,HH-2.5 | x | NA | |
| ABL | 125135 | VpYELMR | Y469 | AA-0.25,GG-2.8,II-3.3 | x | NA | ||
| BCR | 11038641 | HQDGLPYIDDpSPSSpSPHLSSK | S459, S463 | S459:M-4.0,R-0.56,S463:G-1.3,H-1.6,R-0.32 | x | NA | ||
| CAS-L | 8567380 | LpYQVPNSQAASR | Y91 | B-1.5,C-1.0,J-0.56 | x | x | NA | |
| CAS-L | 8567380 | DVpYDVPPSHSTQGVpYDIPPS SVK | Y176, Y188 | Y176:C-0.14,J-0.01,Z-0.04 Y188:C-0.20,J-0.01,Z-0.03 | x | NA | ||
| CBL | 115857 | IKPSSSANAIpYSLAAR | Y655 | Y655 (39) | B-2.2 | x | x | NA |
| CBL | 115857 | LPPGEQGEpSEEDTEpYMTPTS RPVGVQKPEPK | S675, Y681 | Y681 (45) (Y700) | S675:E-3.6,F-0.40,K-1.8 Y681:J-0.08,W-0.04,Z-0.06 | x | x | NA |
| DYRK1A | 6015042 | IYQpYIQSR | Y321 | Y321 (46) | None | x | NA | |
| SHEP1 | 7305329 | AGESpYTHIR | Y273 | None | x | NA | ||
| SHP-2 | 6755228 | VpYENVGLMQQQR | Y584 | Y584 (47) | O-0.55,Y-1.9,JJ-1.9 | x | NA | |
| k562 | ||||||||
| ABL | 125135 | LMTGDTpYTAHAGAK | Y393 | Y393 (43) | None | x | x | |
| ABL | 125135 | LMTGDTpYpTAHAGAK | Y393, T394 | Y393 (43) | Y393:None,Y394:None | x | x | |
| ABL | 125135 | LpYVSSESR | Y185 | None | x | x | ||
| ABL | 125135 | LGGGQpYGEVYEGVWK | Y253 | N-4.0,P-4.0,X-0.20 | x | |||
| ABL | 125135 | LGGGQYGEVpYEGVWK | Y257 | X-0.32,Y-0.65,II-0.83 | x | |||
| Activated p21cdc42Hs | 18553914 | pYATPQVIQAPGPR | Y827 | J-4.6,W-3.0 | x | |||
| Activated p21cdc42Hs | 423137 | VSSTHpYpYLLPERPSYLER | Y912, Y913 | Y912:BB-1.2,GG-2.1,HH-0.04 Y913:C-3.9,J-3.4,DD-1.3 | x | |||
| BCR | 11038641 | PFpYVNVEFHHER | Y177 | Y177 (30) | N-2.7,Q-0.03,BB-3.2 | x | ||
| BCR | 11038641 | HQDGLPYIDDpSPSSpSPHLSSK | S459, S463 | S459:M-4.0,R-0.56,S463:G-1.3,H-1.6,R-0.32 | x | x | ||
| BCR | 11038641 | RLpTWPR | T310 | A-2.4,D-1.9,EE-0.55 | x | x | ||
| BCR | 11038641 | NSLETLLpYKPVDR | Y644 | None | x | |||
| BCR | 11038641 | LASQLGVpYR | Y591 | None | x | |||
| CBL | 115855 | IKPSSpSANAIpYSLAAR | S669, Y674 | Y674 (39) | Y674:B-2.2,S669:None | x | ||
| CBL | 115855 | IKPSSSANAIpYSLAAR | Y674 | Y674 (39) | B-2.2 | x | ||
| Cortactin | 4885205 | LPSSPVpYEDAASFK | Y421 | Y421 (48) | O-1.6,Y-1.3,AA-2.0 | x | ||
| DNABP1 | 4557447 | ELEEIpYMLPR | Y1068 | B-0.37,W-0.66,DD-1.3 | x | |||
| e3b1 | 2245671 | HNSTTSSTSSGGpYR | Y305 | None | x | |||
| GAB1 | 4503851 | SYpSHDVLPK | S266 | D-0.82,E-3.6,I-4.2 | x | |||
| GAB1 | 4503851 | APSASVDSSLpYNLPR | Y259 | C-2.8,J-0.76,Z-2.1 | x | x | x | |
| GAB2 | 18105042 | HNTEFRDSTpYDLPR | Y266 | C-0.65,J-0.56,Z-0.22 | x | |||
| GAGE-3 | 4503881 | STpYYWPRPR | Y10 | B-0.59,C-1.3,J-2.9 | x | |||
| p56dok | 4503359 | GQEGEpYAVPFDAVAR | Y299 | B-0.04,W-0.66,X-0.67 | x | |||
| p62dok | 4503357 | SHNSALpYSQVQK | Y449 | P-3.0 | x | |||
| plakophilin 4 | 4505843 | NNYALNTTATpYAEPpYRPIQYR | Y478, Y482 | Y478:B-0.02,C-0.52,W-1.0,Y482:None | x | |||
| SHC | 284403 | ELFDDPSpYVNVQNLDK | Y317 | Y317 (49) | L-0.47,Q-0.01,BB-0.33 | x | ||
| SHIP-2 | 4755142 | TLSEVDpYAPAGPAR | Y1135 | W-4.3 | x | x | x | |
| SHIP-2 | 4755142 | GLPSDpYGR(PLSFPPPR) | Y1162 | Y1162 (39) | B-0.60,J-0.96,C-2.8 | x | x | |
| SHIP-2 | 4755142 | DTpYAWHK | Y671 | None | x | |||
| SHIP-2 | 4755142 | LpYEWISIDKDEAGAK | Y886 | P-1.1,W-1.5,GG-0.52 | x | |||
| SHIP-2 | 4755142 | NSFNNPApYYVLEGVPHQLLPP EPPpSPAR | Y986, S994 | Y986 (50) | Y986:L-0.20,N-1.7,FF-0.19,S994:None | x | ||
| SYK | 1174527 | EALPMDTEVpYESPpYADPEEIR PK | Y348, Y352 | Y348, Y352 (51) | Y348:C-0.65,W-0.07,Z-0.22 Y352:C-1.9,W-0.18,BB-3.2 | x |
Times are after STI571 treatment. Scansite motifs are described in Table 1. NA, time points not analyzed in the experiments.