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Visible absorption spectra of species involved in the reductive nitrosylation reaction. Standard spectra, pH 7.4, of met-Hb (◊), Fe(II)NO-Hb (○), and Fe(III)NO-Hb (□). The methemoglobin and Fe(II) nitrosyl hemoglobin spectra are from authentic standards; the Fe(III) nitrosyl hemoglobin spectrum was determined by using factor analysis techniques and scaled as described in the text.
