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. 2003 Jan 10;100(2):461–466. doi: 10.1073/pnas.0233287100

Figure 2.

Figure 2

(Left) NO-group mass balance in the reductive nitrosylation of human met-Hb with NO. The total amount of the reaction products, heme-Fe(II)NO, nitrite, and S-nitrosothiol, is presented as a function of the amount of NO introduced. ○, Experimental determinations; solid line, the best linear fit to the experimental points (slope 1.06 ± 0.03; R = 0.990). (Right) Relative yields of ferrous heme nitrosyl products and the S-nitrosothiol plus nitrite products versus the total product yield. ○, Experimental determinations of ferrous heme nitrosyl; solid line, the best linear fit to these experimental points (slope 0.51 ± 0.01; R = 0.996). ●, Experimental determinations of S-nitrosothiol plus nitrite; dashed line, the best linear fit to these experimental points (slope 0.51 ± 0.02; R = 0.992). Amounts are tabulated relative to the amount of heme present in the reaction medium. Product analysis was carried out as described in the text.