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. 2003 Jan 13;100(2):467–472. doi: 10.1073/pnas.0237326100

Figure 3.

Figure 3

Overall view of an atomic model of PLN bound to SERCA1a in a lipid bilayer. α-Helices are represented by cylinders and β-sheets by arrows. Orange, PLN; green, SERCA. The atomic model of PLN was constructed from two NMR structures (1FJK and 1PLP) and refined with molecular dynamics and energy minimization. The model of SERCA was derived from that for the E2 form of rabbit SERCA1a (1IWO); three cytoplasmic domains (A, N, and P) and 10 transmembrane helices (1–10) are marked. PLN domain Ia is α -helical and lies in a groove in the N domain (K400, D557, V607, and N645 are shown) of SERCA1a. Domain Ib is not helical. Domain II is the transmembrane α-helix, running roughly parallel to the M9 helix of SERCA in the groove formed by M2, M4, M6, and M9. The PLN–SERCA complex was embedded in a lipid bilayer generated by molecular dynamics simulation of dioleoyl phosphatidyl choline.