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Schematic illustration of the energy transfer-dependent emission of dATP† associated with the translocation step. Val‡ represents Val-AMP or Val-tRNAIle. Binding of tRNAIle triggers translocation of Val-AMP or Val-tRNAIle from the active site to the editing site with subsequent occupation of dATP† in the vacated active site. Energy transfer-dependent emission (350 nm) from an excited Trp (shown in black) is transferred to dATP†, resulting in emission at 440 nm.
