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Activities for translocation of misactivated valine by mutant and WT enzymes at pH 7.5 and 22°C. Reactions were initiated after the addition of substoichiometric tRNAIle (≈0.5 equivalents) to a near stoichiometric Val-AMP–IleRS complex (0.8:1.0). Translocation of Val-AMP/Val-tRNAIle to the editing site results in the rapid binding of excess dATP† (30 μM) to the active site.
