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AaSvp inhibits basal and 20E-dependent activation of the A. aegypti Vg gene. 0.6Vg-Luc is a Vg-luciferase reporter gene containing 0.6 kb of the 5′ regulatory region of the Vg gene. Drosophila Schneider cells were transfected with 100 ng of 0.6Vg-Luc, along with pAc5-AaSvp (125 ng), pAc5-AaEcR-B (25 ng), and pAc5-AaUSP-B (25 ng) expression vectors, as specified. Cells were treated, where indicated, with 1 μM 20E for 36 h after transfection and incubated at 22°C.
