Figure 2.

AaSvp directly interacts with AaEcR and AaSvp. (A) Mammalian two-hybrid assay. LBDs of AaEcR, AaUSP, AHR38, and AaSvp were individually fused with GAL4 DBD or VP16 AD. CV-1 cells were cotransfected with control reporter pCMV-LacZ and reporter construct pFR-Luc, together with indicated plasmids. Luciferase activity was normalized with LacZ activity. (B) In vitro GST pull-down assay. CDEF domains of AaEcR, AaUSP, AHR38, and AaSvp were fused to GST tag. Fusion proteins were expressed and incubated with ³⁵S-labeled, TNT-expressed, full-length AaSvp. Luciferase was used as negative control.