Figure 3.

AaSvp represses AaEcR–AaUSP-mediated 20E response without DNA binding. Transfections were performed in CV-1 cells with a total of 500 ng of DNA, including 100 ng of 2xDR1-TATA-Luc, 25 ng of AaEcR-B and AaUSP-B each, and indicated amounts of AaSvp, GAL4 DBD-AaSvp LBD, and GAL4 DBD. The amount and type of DNA added to each transfection were balanced by adding empty expression vectors. Relative Luc activities were normalized to β-galactosidase activity. Ponasterone A (1 × 10⁻⁶ M) was added to the medium after transfection, as indicated.