Comparison of morphology and BrdUrd-labeling pattern in the brains of ERβ−/− mice and their WT littermates at E14.5. Embryos received BrdUrd injection at E12.5 and were examined at E14.5. Comparable coronal brain sections (6 μm, paraffin) were processed for Nissl (A–D) and BrdUrd (E and F) staining. At E14.5, the morphology is similar in the ERβ−/− (B and D) and control (A and C) brains. (C and D) Higher-power views of the boxed areas in A and B, respectively. vz/svz, ventricular zone/subventricular zone; iz, intermediate zone. At E14.5, BrdUrd-labeling patterns are also similar in the telencephalon of the ERβ−/− (F) and control (E) mice. (Scale bars: A and B, 12.5 μm; C–F, 50 μm.)