Figure 6.

Abnormalities in radial glia in ERβ−/− mice. Antinestin antibodies were used to visualize radial glial cells. The radial scaffold formed by processes of radial glia are apparently truncated and appear as many short fragments, with more abundant end feet (*) in layer I in ERβ−/− brains (B and D) compared with their WT littermates (A and C). (C and D) Higher-power views of the boxed areas in A and B, respectively. More astroglial-like nestin-immunoreactive cells (arrow) appeared in ERβ−/− brains (E). (Scale bars: A and B, 50 μm; C and D, 25 μm; E, 5 μm.)