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Colocalization of molecular chaperones, tau, and microtubules. (a) Coimmunoprecipitation of tau with Hsp90 (Left) and Hsp70 (Right) from COS-1 cells, (+) anti-tau Ab, (−) control Ab. (b) Colocalization by triple immunofluorescence microscopy of Hsp70, tau, and microtubules. (Upper) Immunofluorescence staining of COS-1 cells expressing the shortest tau isoform (T44). (Lower) Immunofluorescence staining of COS-1 cells expressing the longest tau isoform (T40). (Bar = 10 μm.)
