Figure 6.

Hsp70 and Hsp90 mediate tau microtubule partitioning/aggregation in a cell-free system. Permeabilized reconstituted cells were incubated for 90 min (Left) or 180 min (Right) and then fractionated as described in Fig. 4a. Membrane-bound tau refers to supernatant 2. “+Hsp70” and “+Hsp90” refer to added recombinant chaperones; “−Hsp70/90” refers to cytosol prepared from siRNA-transfected cells. Arrowheads indicate the absence of phosphorylated tau.