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. 2003 Jan 6;100(2):727–732. doi: 10.1073/pnas.242721899

Figure 3.

Figure 3

Diffusible molecules influence MGE cell migration. (A) Coculture of MGE reaggregate with hypothalamic explant. More MGE cells migrate distally than proximally to the explant. (B) Coculture of MGE reaggregate with neocortical explant. More MGE cells migrate proximally than distally. (C) Quantification of the difference in the number of cells found in the distal and proximal quadrant (percentage difference ± SD). (D) Quantification of the total number of MGE cells migrating out of the explant (number of migrating cells ± SD). (E) In situ hybridization reveals strong expression of Slit1 in the hypothalamic region (arrow) next to the MGE. Slit1 is also weakly expressed in cortical plate (arrowhead), the target area of MGE cell migration. (F) In situ hybridization reveals strong expression of Slit2 in the hypothalamic region (arrow). (G) Mixing of neocortical cells with HEK293 cells expressing Slit1 or Slit2 does not result in suppression of MGE cell migration in the spot assay (the inhibitory activity of Slit-expressing cells was confirmed in coculture assays) (number of cells per spot ± SD). NCx, neocortex; HT, hypothalamus.