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. 2003 Jan 13;100(2):745–750. doi: 10.1073/pnas.0236364100

Figure 6.

Figure 6

Effects of oleoyl-CoA and phosphoinositides on mutant Kir2.1 channels. Inside-out macropatch measurements on recombinant Kir channels expressed in Xenopus oocytes were performed as in Fig. 1. The traces show currents at −80 mV after channel rundown. (A) Representative trace for Kir2.1(K185Q) channels. The applications of 25 μM diC8 PI(4,5)P2 and 25 μM diC8 PI(3,4,5)P3 are shown by the horizontal lines before and after the application of 10 μM oleoyl-CoA. (B) Similar measurement as in A with the triple-mutant Kir2.1(D51K-I79L-K185Q) channels. (C) The effect of 10 μM oleoyl-CoA on the triple-mutant of Kir2.1 before and after 5 μM AASt PI(4,5)P2. Twenty-five micromolar diC8 PI(4,5)P2 was applied at the beginning of the experiment.