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Time course of berberine influx into oocytes injected with Cjmdr1 mRNA. Cjmdr1- and water-injected oocytes were incubated in berberine medium (1 mM) and sampled at the times indicated for transfer into the lysis buffer. The lysate was lyophilized and dissolved in 50% methanol to be analyzed by HPLC with a photodiode array detector. The quantity of berberine was calculated from the peak area.
