Table 1.
Sequences of the peptides from TSP9
| No. | Sequence | Theoretical mass | Experimental mass |
|---|---|---|---|
| 1 | AAKGTAETKQEKSFVDWLLG | ||
| 2 | SFVDWLLGK | 1,063.58 | 1,063.53 |
| 3 | ITKEDQFYETDPILR | 1,866.94 | 1,866.86 |
| 4 | EDQFYETDPILR | 1,524.71 | 1,524.66 |
| 5 | KDGDGGVFGGLFAK | 1,366.70 | 1,366.65 |
| 6 | DGDGGVFGGLFAK | 1,238.60 | 1,238.55 |
| 7 | GGDVK | 474.25 | 474.25 |
| 8 | GGtTSGK | 686.27 | 686.31 |
| 9 | KGtVSIPSK | 995.51 | 995.51 |
| 10 | SSGStSGK | 789.30 | 789.33 |
The N-terminal sequence (peptide 1) was obtained by Edman degradation. Peptides 2–10 from TSP9 digested with trypsin have been sequenced by using collision-induced fragmentation mass spectrometry. A single-letter amino acid code is used; the lowercase t designates phosphothreonine residues. The shown peptide masses are monoisotopic.