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Diagram of the baculovirus expression constructs for expression of soluble, six-histidine-tagged (6xH) truncated HCoV-229E spike glycoprotein (S). The number next to the S indicates the amino acid at which the construct was truncated. Each construct contains the HCoV-229E signal sequence. The region corresponding to the TGEV receptor-binding domain was identified by Godet et al. (14).
