Figure 2.
Transcriptional activities of MPA, R5020 and DHT under the control of PR and PR isoforms. (a) T47Dco breast cancer cells with wild-type equimolar levels of PR-A and PR-B. T47Dco cells were transfected with PRE2/luciferase and a Renilla luciferase internal control. Cells were treated for 20 hours with MPA, R5020, or DHT, at concentrations ranging from 0.01 to 1000 nmol/l. Luciferase activity was quantitated as a percentage of 1 μmol/l R5020 activity. The study was performed in triplicate and data are reported as mean ± standard error. *P < 0.05, 1 μmol/l dose of hormone versus 1 μmol/l R5020. (b) HeLa cervicocarcinoma cells stably expressing PR-A. (c) PR-negative T47D-Y breast cancer cells transiently transfected with PR-A. (d) HeLa cells stably expressing PR-B. (e) T47D-Y cells stably expressing PR-B. Transient transfection and transcription studies were performed as described above for panel a. DHT, dihydrotestosterone; MPA, medroxyprogesterone acetate; PR, progesterone receptor.