FIG. 4.

Longitudinal tracking of Env-specific Th cells from subject 1212. PBMC from six time points between 16 DPI and 27 months posttreatment (weeks 0, 24, 40, 78, 104, and 120 posttreatment) were tested in a batch analysis for lymphoproliferation (S.I. are shown) and IFN-γ secretion by ELISA in response to stimulation with peptide PI20 for 120 h. PBMC from these time points were also stimulated with the peptide for 10 days, and IFN-γ ELISPOT assays were performed with CD8-depleted PBMC poststimulation. Env-specific IFN-γ SFC were detected by stimulated ELISPOT only.