FIG. 2.

The 5′-terminal nucleotide sequence determined from genomic RNA. RNA was purified from virions and sequenced by the dideoxy method. For each virus, two sets of sequencing reactions are shown. In the set on the left (−), the reaction products were not further treated. In the set on the right (+), the reaction products were further treated with terminal deoxynucleotidyl transferase. (A) Wild-type virus. (B) L30-12.2 virus. (C) A30-1.2 virus. (D) A40-1.1 virus. (E) M40-1.1 virus. (F) Virus rescued after transfection with transcripts containing Met-nsP4 and a GAUU extension at the 5′ terminus. The arrows indicate the A residue corresponding to the 5′-terminal A of the wild-type virus.