TABLE 2.
Effect of adding or modifying nucleotides at the 5′ terminus of the genomic RNA on SINV mutants with altered amino acids at the N terminus of nsP4
| Virus | 5′ terminus of Transcript RNAa | N-terminal amino acid of nsP4 | Plaque sizeb after transfection at 30/40° | 5′ terminus of RNA from rescued virus | Plaque size after infection at 30/40°C |
|---|---|---|---|---|---|
| Wild type | ·GAUUGAC | Tyr | L/L | ·AUUGAC | L/L |
| Mutants | ·GUAU------ | Tyr | L/L | ·------ | L/L |
| ·GUAU------ | Ala | S/Min | ·AU------ | S/S | |
| ·GAU------ | Ala | S/S | ·AU------ | S/S | |
| ·GA--A--- | Ala | S/none | ·--A--- | S/Min | |
| ·GAUU------ | Met | S/S | ·AUU------ | S/S | |
| ·G--A--- | Leu | S/Min | ·--A--- | S/Min | |
| ·G--A--- | Arg | S/Min | ·--A--- | S/Min | |
| ·GG----- | Leu | None/none | |||
| ·GG----- | Arg | None/none |
a
·, cap structure; −, same nucleotide as in the wild type.
b
L, large plaques; S, small plaques; Min, minute plaques.