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. 2006 Mar 1;20(5):613–623. doi: 10.1101/gad.1345006

Figure 1.

Figure 1.

Jab1 interacts with the E2F1 marked box domain. (A) Structure of two-hybrid bait proteins. Graphical representation of bait plasmids used to identify E2F1-specific two-hybrid-interacting proteins. (DBD) Yeast GAL4 DNA-binding domain; (L) 14-amino-acid linker separating the DBD from the E2F bait; (MB) “marked box” regions of E2F1 or E2F3; (Adjacent) region between the marked box and C-terminal transactivation/Rb-binding domain. Amino acids 252–386 of E2F1 and 305–410 of E2F3 were used in these two-hybrid fusion genes. (B) Two-hybrid interaction assays. Jab1, HSP86, and A-FABP are three of the 12 clones recovered from the two-hybrid library as E2F1-interacting proteins. “2-hybrid interaction” refers to the comparative strength of growth on selective media of diploid strains containing either GAL4-E2F1, GAL4-E2F3, or GAL4-Cdc6 and the library-interacting plasmid.