TABLE 1.
Bioassay in TgOv mice of VRQ- and ARR-expressing Rov cells challenged with sheep prion
| Ovine PrP allele | Infected culturea
|
Mean survival time (n/n0)b ± SD | ||
|---|---|---|---|---|
| Clone | Passage p.i. | Dilution | ||
| VRQ | Rov9 | 14 | 1/106 | 89 ± 2.4 (5/5) |
| VRQ | Rov9 | 14 | 1/107 | 104.5 ± 2.1 (2/5) |
| VRQ | Rov9 | 14 | 1/108 | >300 (0/5) |
| VRQ | RovA7 | 8 | 1/1 | 57.3 ± 0.73 (4/4) |
| ARR | RovE2 | 8 | 1/1 | >420 (0/5) |
| ARR | RovG4 | 8 | 1/1 | >450 (0/7) |
| None | NAc | 8 | 1/1 | >420 (0/6) |
a
Mice were inoculated intracerebrally with 20 μl of cell extracts from infected Rov or parental RK13 cultures at the indicated passage postinoculation, either undiluted (3 × 106 cells per mouse) or diluted as indicated. Two distinct infected VRQ- and ARR-Rov clones were bioassayed. The titer of infected Rov9 culture, determined according to Kärber's method, was 2.65 LD50 per cell (i.e., 106.9 LD50 in 3 × 106 cells).
b
Mean days to the death ± SEM; n/n0, number of terminally ill/number of inoculated animals.
c
NA, not applicable.