FIG. 3.

Western blot analysis of incorporation of SeV wild-type and mutant F and HN proteins into SIVagm vectors. Virion lysates of SeV wild-type (wt) and mutant F- and HN-pseudotyped vectors were prepared from pellets of supernatants of producer cells by ultracentrifugation. Samples were transferred to a polyvinylidene difluoride membrane and blotted with the anti-HN antibody HN-2 (A) or the anti-F1 antibody (B). SeV virion lysates were used as the positive control for each protein.