FIG. 5.

Inhibition of transduction by SeV mutant F- and HN-pseudotyped SIVagm vector with a neutralizing antibody specific to SeV. The SeV Fct4- and SIVct+HN-pseudotyped vector at 0.8 × 10⁴ T.U. or the VSV-G-pseudotyped vector at 1.2 × 10⁶ T.U. was incubated with the immunoglobulin G fraction of a neutralizing antibody specific to SeV (anti-HN antibody HN-2) or VSV (anti-VSV-G antibody I-1) at 4°C for 30 min, followed by transduction of 293T cells. Seventy-two hours after the transduction, EGFP-expressing cells were detected by fluorescence microscopy.